Katsuhide Mabuchi, Ph.D.

Katsuhide Mabuchi, Ph.D.

Senior Research Associate

Heart disease
kmabuchi@bbri.org

Research Summary

Phosphorylation of proteins are involved in the regulation of enzyme activity, enzyme substrate specificity and formation of functional multi-unit complexes. One enzyme, PP1c delta protein phosphatase removes a phosphate from many different phospho-proteins. Very uniquely, the enzyme can form complexes with different regulatory subunits to become more or less specific to one phosphoprotein. In the case of myosin light chain phosphatase, the delta catalytic enzyme is complexed with myosin targeting and the second small proteins forming a trimeric holoenzyme. We are studying how the non-catalytic subunits alter activity and substrate specificity under the directorship by Dr. T. Tao. In addition, I use an electron microscopic method to visualize individual protein molecules. The method is particularly suited to examine variation among different molecules.

 

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Selected Publications

  • Tokuo H, Mabuchi K, Ikebe M. (2007) The motor activity of myosin-X promotes actin fiber convergence at the cell periphery to initiate filopodia formation. J Cell Biol 179(2), 229-38.
  • Li, X. D., Jung, H. S., Mabuchi, K., Craig, R. & Ikebe, M. (2006). The globular tail domain of myosin Va functions as an inhibitor of the myosin Va motor. J Biol Chem 281, 21789-98.
  • Watanabe, S., Mabuchi, K., Ikebe, R. & Ikebe, M. (2006). Mechanoenzymatic characterization of human myosin Vb. Biochemistry 45, 2729-38.
  • Okamoto, R., Kato, T., Mizoguchi, A., Takahashi, N., Nakakuki, T., Mizutani, H., Isaka, N., Imanaka-Yoshida, K., Kaibuchi, K., Lu, Z., Mabuchi, K., Tao, T., Hartshorne, D. J., Nakano, T. & Ito, M. (2006). Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart. Cell Signal 18, 1408-16.
  • Tan, Y., Chen, M., Li, Z., Mabuchi, K. & Bouvier, M. (2006). The calcium- and zinc-responsive regions of calreticulin reside strictly in the N-/C-domain. Biochim Biophys Acta 1760, 745-53.
  • Ikebe, M., Li, X. D., Mabuchi, K. and Ikebe, R. (2005). Conformational change and regulation of myosin molecules. Adv Exp Med Biol 565, 61-72; discussion 72, 359-69.

 

PubMed:
Click here for a list of publications (searches the National Library of Medicine's PubMed database.)

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